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gamma irradiated drug resistant dr4 irmef feeder cells  (ATCC)


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    ATCC gamma irradiated drug resistant dr4 irmef feeder cells
    Gamma Irradiated Drug Resistant Dr4 Irmef Feeder Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/MEF/bio_rxiv__2025__07__16__665154-199-28-34
    Average 96 stars, based on 253 article reviews
    gamma irradiated drug resistant dr4 irmef feeder cells - by Bioz Stars, 2026-11
    96/100 stars

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    Article Title: Xist Repeat A coordinates an assembly of SR proteins to recruit SPEN and induce gene silencing
    Article Snippet: Starting around 24 h after transfection and lasting at least 5 days, cells were selected in growth medium containing 2 μg/mL puromycin dihydrochloride (Corning 61-385-RA) and 200 μg/mL G418 sulfate (Thermo Fisher 10131035).

    Transfection:

    Article Title: Xist Repeat A coordinates an assembly of SR proteins to recruit SPEN and induce gene silencing
    Article Snippet: The following day, cells were given fresh growth medium and transfected with a plasmid mixture containing 2 μg PX459_v2-Srsf1-N1-gRNA and 2 μg pGW-Amp-Srsf1-N_term-dTAG-HDR using Lipofectamine 3000 (Thermo Fisher L3000001). .. One day after transfection, all cells were split and seeded onto a confluent 10-cm dish of gamma-irradiated DR4 MEF feeder cells (ATCC SCRC-1045). .. The following day, cells were given fresh growth medium and transfected with a plasmid mixture containing 1.5 μg pCL116-EGFP-Halo or pCL116(PuroR)-Halo-SPEN-V5 (WT or SPEN mutant) and 0.5 μg pUC19-piggyBac transposase using Lipofectamine 3000 (Thermo Fisher L3000001).

    Article Title: Xist Repeat A coordinates an assembly of SR proteins to recruit SPEN and induce gene silencing
    Article Snippet: .. Three days after transfection, cells were split and seeded sparsely (500, 1500, 3000, or 6000 cells) onto confluent 10-cm dishes of gamma-irradiated DR4 MEF feeder cells (ATCC SCRC-1045). ..



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